Metrohm 833 IC Liquid Handling Unit - Manual (Page 70)

7 833 IC Liquid Handling Dialysis Unit
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
62
Conditioning the dialysis system
Before the first analysis the dialysis cell with the inserted dialysis
membrane and all the tubing connections must be rinsed with ultrapure
water. Proceed as follows:
1 Settings at 820 IC Separation Center
• Switch injection valves A and B in the 820 IC Separation
Center to the setting "FILL" in the «IC Net» software.
2 Start-up the 833 IC Liquid Handling Dialysis Unit
• Immerse the 2 pieces of aspiration tubing 16 (see Fig. 21) in
the acceptor solution (degassed ultrapure water).
• Switch on 833 Liquid Handling Pump Unit and 833 IC Liquid
Handling dialysis Unit with mains switch 8.
• Start the peristaltic pumps in «IC Net» by setting the remote
line 1 (833 Pump Unit) and remote line 2 (833 Dialysis Unit) in
the window of the 819 Detector.
• Set the contact pressure for both cassettes: press contact
lever 5 until the solutions are just aspirated. Then press the
contact lever up by 1 further click to achieve the optimum
contact pressure.
• Rinse the dialysis system for approx. 10 min with ultrapure
water and check that the same amount of solution emerges
from both the waste outlets in the waste bottle.
3 Conditioning the dialysis membrane
• Rinse the dialysis system for approx. 20 min with ultrapure
water. Check that the same amount of solution emerges from
both the waste outlets in the waste bottle.
• Check all tubing from the storage containers to the tubing
cassettes and dialysis cell and up to the waste bottle for
leaks. If any leaks are found then the corresponding
connection must either be tightened up or replaced.
• If any air bubbles remain in the dialysis cell then screw off
PEEK tubing 62 (acceptor solution) and PTFE tubing 58
(sample) from outlets 49 and 52 of the dialysis cell and wait
until the air bubbles have vanished. Then screw the tubing
back on again.