Metrohm 833 IC Liquid Handling Unit User Manual
Page 73
7.6 Optimizing the dialysis
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
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1 Measure standard directly
•
Immerse the aspiration tubing 16 of the acceptor solution in
the required ionic standard. This should have approximately
the same total ionic concentration as the sample.
•
Start a determination in «IC Net» to measure the standard
directly; the program could look like that shown below:
•
Immerse acceptor solution aspiration tubing 16 (see Fig.
21)in the acceptor solution again and rinse the acceptor
channel for approx. 2 min.
•
Switch pump of the 833 IC Liquid Handling Dialysis Unit off.
2 Dialyze the standard and measure it
•
Set the dialysis time to 5 min in the program (see
•
Immerse sample aspiration tubing 16 in the standard used in
item 1.
•
Start the determination in IC Net and wait until the
chromatogram has been evaluated.
3 Further measurements
•
Increase the transfer time in the program (see
Section 7.3.1/7.3.2) in steps of 5 min until the measured
values are constant.
•
Carry out measurements as described in item 2.
4 Determine the optimal dialysis time
•
Plot the relationship between the measured peak areas or
concentrations as a function of the dialysis time (i.e. the
recovery rate) and determine the optimal dialysis time.
Not for dialysis without suppression