Metrohm 833 IC Liquid Handling Unit - Manual (Page 55)

6.2 Connection to modular IC system for cation separation
833 IC Liquid Handling Unit / Instructions for Use 8.833.1003
47
• Immerse the other end of the aspiration tubing in a container
with rinsing solution (normally deionized water) and fix it
position.
• Pull outlet capillary 39 (marked with “Waste”) at sample
preparation module connection 34 (see Fig. 13) through one
of the openings 18 in the rear panel of the 820 IC Separation
Center.
• Lead outlet capillary 39 into a sufficiently large waste bottle
and fix it in position.
8 Start-up 833 IC Liquid Handling Sample Preparation
Unit
• Switch on 833 LH Pump Unit with mains switch 8.
• Set the contact pressure for both cassettes: press contact
lever 5 until the solutions are just aspirated. Then press the
contact lever up by 1 further click to achieve the optimum
contact pressure.
• Check all the tubing from the storage containers to the 833 IC
Liquid Handling Unit and the sample preparation module
through to the waste bottles for leaks. If any liquid should be
leaking then the corresponding connection must be tightened
up or replaced.
• Before the sample preparation module is used for the first
time the cation exchanger material must be conditioned. This
is done by injecting e.g. 30% NaOH 15 times (5 injections per
unit). You can use the program described in the following
section for the system “Neutralisation.smt” with a running
time of 20 min; the column does not need to be mounted.
6.2.2 Settings in IC Net
When setting up the 833 IC Liquid Handling Sample Preparation Unit
and the 833 IC Liquid Handling Unit the “New system wizard” of the IC
Net program demands the assignment of an “Event line” for the
Actuator, i.e. the sample preparation module. Although this does not
exist you should still select any unused Event line so that the “New
system wizard” can continue. In fact the sample preparation module is
controlled from the 766 IC Sample Processor (see Fig. 14).
The signal for switching the internal suppressor module of the 820 to
the next position is not controlled by the program of the 766 IC Sample
Changer. Instead the parameter “Auto Step” is set to “fill” in the
parameter window of the 820 IC Separation Center in IC Net (double-
click on 820 symbol).