UVP Doc-It Life Science - Manual (Page 98)

Performing Colony Counting
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Circle Radius
To change the circle radius, click the drop down arrow and select from the numbers to change
circle pixel radius.
Text Annotation
To change the text annotation behavior, select from:
Synchronize size with image zoom: Increases/decreases the size of the annotation if
the size of the image changes using the zoom feature.
Don t synchronize with image zoom: Does not increase/decrease the size of the
annotation if the size of the image changes while using the zoom feature.
Log Preferences
The Logging preferences window allows users to define the log file path and the log level.
To change the Log File Path , click … and locate a new directory.
To change the Log Level, click the drop down arrow and select from the list.
Automatic Counting
Open the Colony Counting Plug-in by selecting View > Plug-in > Colony Counting Plug-
in and select Start Colony Count.
Select Automated Count.
Select OK.
The automated count will be displayed.
Colonies can be filled in with red or outlined. Colonies can also be numbered or
annotated using multiple colors.
If desired, colonies can added, split, deleted, merged.
Manual Counting
Open the Colony Counting Plug-in by selecting View > Plug-in > Colony Counting Plug-
in and select Start Colony Count.
Select Manual Count.
Click OK.
The Manual Count Colony Wizard module will open.
Proceed to Step 1 and Step 2 of the manual counting process.
Manual Counting Step 1: Select Classes
Manual Counting Step 2: Finish