UVP Doc-It Life Science - Manual (Page 74)

Performing 1D Analysis
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In this window, the software allows users to perform various changes to the lane including:
Name
Geometry
Band Properties also offers the following information:
Band ID
Left and Right band positions
Calculated Peak Values (Rf value and Molecular Weight);
The Intensity of the band, including its Maximum, Volume, % of lane, and Mass; and
The Concentration of the band, including its Maximum, Volume, % of lane, and Mass.
Lane Profile Graph
LS software allows users to view profile graphs (intensity vs. position) of one or more of the lanes in the
image in the Lane Profile Graph. To access this function, select Lane Profile Graph from the Analysis >
1D Analysis > Master tools > Lane Profile Graph or click on Lane Profile Graph from the 1D Analysis
plugin. A new window appears.
Underneath the graph, the software displays an image of the graphed lane (or of the lane last selected to
be graphed).
In this section, the following topics are presented:
How to display a single lane
How to display multiple lanes at one time
How to change variables for the x-axis and the y-axis
How to change colors of the graph
How to display specifics including band extents, band peaks, and background correction
Display a Single Lane
There are two options for displaying a single lane.
To Display a Single Lane by Selecting a Lane
Select the lane of interest.
From the Analysis > 1D Analysis > Master Tools select Lane Profile Graph or click on Lane
Profile Graph from the 1D Analysis plug-in. A new window appears with a graph of the
selected lane.
To Display a Single Lane from the Lane Profile Graph
From the Analysis > 1D Analysis > Master Tools, select Lane Profile Graph or click on Lane
Profile Graph from the 1D Analysis plug-in. A new window appears with an empty graph.
From the Lanes section of the Lane Profile Graph window and to the right of the graph, click on
the lane to graph (Lane A is selected in the example on the right). The software automatically
displays the graph of that lane.