UVP Doc-It Life Science - Manual (Page 79)

Performing 1D Analysis
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I-Max: Maximum intensity value reached within a lane. It actually shows the brightest (for black
background) or darkest (for white background) pixel value in the specific lane, with values
accordingly with the image’s bit depth.
I-Vol: Sum of all intensities found in every pixel of the current lane.
C-Max: Highest concentration value for a pixel achieved in this lane. It is computed accordingly
with user-selected concentration.
C- Vol: Sum of each pixel’s concentration from the selected lane.
LanesBands: Number of bands found in the current lane.
Bands for Each Lane Report
Lane ID: ID of the referenced lane.
Band ID: ID of the current band.
Name: Given name by editing.
MW: Computed MW value after a MW calibration has been applied.
Rf: Computed Rf value obtained either by default assigning 0 to top and 1 to bottom, user-
defining Rf lines, or applying MW calibration.
Top: Intensity of the higher extent.
Peak: Max intensity of the extent.
Bottom: Intensity of the lower extent.
Left: Position of the left extent.
Right: Position of the right extent
I-Max: Maximum intensity value reached within a band. It actually shows the brightest (for black
background) or darkest (for white background) pixel value in the specific band, with values
accordingly with the image’s bit depth.
I-Vol: Sum of all intensities found in every pixel of the current band.
I- %: Lane’s volume percentage occupied by this band’s volume.
NOTE: I% of bands will sum up to 100% only if when the option "Lane volume is sum of bands" is
checked. Otherwise, the lane volume value will include all pixels, including those outside the bands.
I-Mass: How much of the user-calibrated mass for the lane this band is accounted for.
C-Max: Concentration represented of the maximum value in a band.
C-Vol: Sum of concentration in one band.
C%: Percentage concentration in one band.
C-Mass: Amount of reagent in one band with respect to the amount in the entire lane.
Background Correction: Value that defines the background correction applied to the current band
as follows: for white backgrounds, the higher the value, the lesser the correction applied; for black
backgrounds, the lower the value, the lesser the correction.
Concentrations Report
Each row represents the number of the band and each column represents the ID of the lane.