UVP Doc-It Life Science - Manual (Page 103)

Performing Colony Counting
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In the Spiral Plate Analysis window move the Overlay Size slider bar to the left (to
decrease the grid size) or to the right (to increase the grid size).
To calculate the SPLC/mL:
Type in or use the up or down arrows in the Total volume deposited box provided. The
calculated amount will appear immediately below the Calculate SPLC/mL button.
View Results
To view the resulting Data Table analysis for the spiral plate count:
Click onto the + sign (expanded report shown here after clicking the + sign).
The values listed will provide the number of colonies found in each quadrant and section
of the counted plate.
To save the spiral plate settings in a template:
Click onto Save As and provide a name for the template.
To print or export the data into Excel:
Click onto the Print or Export to Excel button.
User Tips
While in the Spiral Plate Analysis window, the user may choose to move the green overlay grid
by using the Align Spiral Plate Overlay.
To move the grid:
Click onto Align Spiral Plate Overlay
Click and drag the green grid until the center of the grid is aligned with the center of the
captured image.
To clear the spiral plate analysis:
Click onto the Clear Spiral Plate Analysis. This action will remove the data entered for
this plate and remove the spiral plate grid.
Spiral plate analysis may be performed on any counted plate. To perform the spiral plate
analysis on the previously counted plate:
Select the Spiral Plate Analysis option directly above the Align Spiral Plate Overlay
option.
Zone Analysis
Zone analysis provides users with zone sizing information useful for applications such as
inhibition zone analysis.
Before proceeding through the zone analysis process, ensure that an image of the Petri dish has
been captured and is ready for analysis.
To obtain zone analysis information for a plate:
Click on the checkbox for Zone.
Select Start Colony Count.
Choose to count with the Manual or User Defined method.