Ocean Optics S2000 - Manual (Page 73)

Checking the Alignment
1. With the spectrometer running, observe the signal in
Scope Mode. When the optical window is properly
positioned, you can see a full UV transmission through
the cell. If the polyimide is in the optical path, you
will see just the red end of the spectra. If this occurs,
loosen the fittings and slide the sample tube to align
with the window until you achieve the best signal (on
both the wavelength and intensity axes).
2. If the fibers are not properly inserted in the through-
hole, the intensity will be low. To maximize intensity,
loosen the fiber fittings and adjust the fiber.
3. When the fibers and sample tubing are perfectly
aligned, make sure all fittings are snug.
4. Mount the cell in your apparatus using the mounting
holes. It is important that the optical fibers are not
moved during measurements. If necessary, secure the optical fibers to relieve stress, especially where the fibers
connect to the cell.
Performing CUV-CCE Measurements in OOIBase32
1. Make sure you are in Scope Mode. Select boxcar smoothing and signal averaging values and an integration
period that won't saturate the detector.
2. While still in Scope Mode, take a dark spectrum by first disconnecting the optical fiber from the lamp. Take
the dark reading by clicking the store dark spectrum icon on the toolbar or selecting Spectrum | Store Dark
from the menu.
3. Fill the tube with the blank solution or solvent. The peak intensity of the reference signal should be about 3500
counts. Take a reference spectrum by first making sure nothing is blocking the light path going to your sample.
Take the reference reading by clicking the store reference spectrum icon on the toolbar or selecting Spectrum |
Store Reference from the menu.
4. Reconnect the optical fiber to the lamp.
5. Switch from Scope Mode to Absorbance Mode.
6. The data can viewed as a time series of values from a single wavelength, an integrated band around a
wavelength, or a mathematical combination of wavelengths. Consult the directions in the
OOIBase32
Spectrometer Operating Software Manual for using the time series functions.
Specifications
Sampling Chambers: CUV-CCE
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Dimensions: 1.09" x 1.09"
Maximum fused silica tubing allowed: 500- µ m in diameter
ID tubing sleeves: F230 0.016"
Cell fixture: 10-32 PEEK Cross (Upchurch # P-729)
Through-hole size: 0.020"
Coned female threads: 10-32
Fingertight fittings: F-300 PEEK
UV waveguide optical fibers:
Al-jacketed, 300- µ m diameter, solarization-resistant, silica-
core/silica-clad
Path length: silica tubing diameter
Size of light beam reaching sample: ~5 mm (circular)