Ocean Optics S2000 - Manual (Page 46)

R-LS-450 Rack-mount Blue LED Pulsed Light Source
The R-LS-450 B LUE LED P ULSED L IGHT S OURCE is a compact, low-cost light-emitting diode that produces pulsed
or continuous spectral output at 470 nm -- the blue region -- for high-sensitivity emission fluorescence
measurements. The R-LS-450 is the rack mount version of the LS-450. The R-LS-450 can be configured to operate
in continuous wave mode through manual operation and through the software. It can also be configured to operate in
pulsed mode through manual operation and through the software.
! !
The R-LS-450 is shipped with the following pins jumpered: Jumper over pins in JP1, a jumper over the Manual
pins in JP3, and a jumper over the 2
10 pins in JP2 for the fastest pulse rate available.
Operation with the R-LS-450 Board
You can configure the lamp’s performance through a switch and three jumper blocks on the circuit board of the
R-LS-450 and, if desired, through one jumper block on the circuit board of the S2000 spectrometer. The following
lists the many different choices you have for configuring the R-LS-450 for your application. You need to determine
the best mode of operation for your setup and configure your system appropriately.
S1 Switch
The S1 Switch is a three-position switch on the R-LS-450. The switch can be positioned in continuous wave
operation, no operation, and pulsed operation.
Jumper Block 1 (JP1)
There is only one set of pins in JP1. If other jumper blocks are configured correctly, a jumper over JP1 allows you to
turn the R-LS-450 on and off via the Strobe Enable feature in OOIBase32 software and to even control the pulse rate
through the Flash Delay feature in OOIBase32 software. This feature is only available with a “J” or later version of
the S2000. (To find out if you have a J-series or later S2000,.see the third letter in your S2000 serial number).
Jumper Block 2 (JP2)
There are nine sets of pins in JP2. The number of pulses per second of the R-LS-450 depends on the pins you
jumper on JP2. However, the pulses per second are also dependent upon the master frequency of your A/D converter.
" A jumper over the CW pins makes the R-LS-450 continuously on, which means that there is no pulsing of the
light source. Other jumper blocks must be configured correctly.
" A jumper over the 2 16 , 2 15 , 2 14 , 2 13 , 2 12 , 2 11 and 2 10 pins controls the pulse rate per second of the R-LS-450,
depending on the A/D converter you are using to interface to your S2000. (See table below for pulse rates.)
" A jumper over the CS pins allows you to control the pulse rate via the OOIBase32 software. (See Using JP3 on
the S2000 on the next page for more information.)
- 46 -
Light Sources: R-LS-450
Pins on the JP2 Function
DAQ700
Frequency (Hz)
ADC500 / SAD500
Frequency (Hz)
ADC1000
Frequency (Hz)
CW Continuous Mode 0 0 0
2
16
Divide by 2
16
1.5 7.6 15.2
2
15
Divide by 2
15
3.1 15.2 30.4
2
14
Divide by 2
14
6.1 30.0 60.8
2
13
Divide by 2
13
12.2 60.8 122.0
2
12
Divide by 2
12
24.0 122.0 244.0
2
11
Divide by 2
11
48.0 244.0 488.0
2
10
Divide by 2
10
98.0 488.0 976.0
CS* Continuous Strobe N/A N/A Software Controlled