Ocean Optics S2000 - Manual (Page 58)

CUV-ALL 4-way Cuvette Holder
The CUV-ALL 4- WAY C UVETTE H OLDER for 1-cm path length cuvettes has four collimating lenses that couple to
optical fibers, light sources, and spectrometers to measure absorbance, fluorescence, scattering, or any combination
of these optical phenomena.
Parts Included
" CUV-ALL assembly for holding 1-cm cuvettes
" Black Cover for eliminating ambient light
" Two Barbed Fittings for connecting a temperature stabilizing water source
" Screwdriver for adjusting the fit of the cuvette
" Allen wrench for adjusting the collimating lenses
Operation
Attaching the Fibers for Absorbance Measurements
1. For UV measurements, attach one end of a SMA-terminated optical fiber to one of the collimating lenses with
the UV label and a red lens cap. Attach the other end of this fiber to a UV light source. Attach another SMA-
terminated optical fiber to the collimating lens directly opposite of the first fiber. It too has a red lens cover.
Attach the other end of this fiber to the spectrometer.
2. For VIS measurements, attach one end of a SMA-terminated optical fiber to one of the collimating lenses with
the VIS label and a black lens cover. Attach the other end of this fiber to a light source. Attach another SMA-
terminated optical fiber to the collimating lens directly opposite of the first fiber. It too will have a black lens
cover. Attach the other end of this fiber to the spectrometer.
Attaching the Fibers for Fluorescence Measurements
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses. Attach the other end of this
fiber to a light or excitation source. Typically, for fluorescence measurements, the illumination fiber connects
to a UV lens.
2. Attach another SMA-terminated optical fiber to the collimating lens to the immediate left or right of the first
fiber. The two collimators must be positioned at 90° for fluorescence measurements. Attach the other end of
this fiber to the spectrometer. Typically, for fluorescence measurements, the read fiber connects to a VIS lens.
Using the Fiber Supports
1. Snap the clamps around the fibers after the fibers are screwed into the light source and spectrometer.
2. Lift the clamps until they support the fibers.
3. Unsnap the clamps to remove the fibers.
Adjusting the Fit of the Cuvette
The CUV-ALL is preset for use with a 1-cm quartz cuvette. When properly adjusted, the cuvette should fit snugly
in the holder.
1.
Locate the two ball plunger screws. They are under two of the four collimating lenses.
2. Remove the fiber clamps for easier access to the screws.
3. Use the provided screwdriver to loosen the two ball plunger screws until the ball end of the screws is just
visible in the cell holder.
4. Insert your cuvette into the holder.
5. Tighten the ball plunger screws until the ball contacts the cuvette and starts to compress. Do not over-tighten.
Sampling Chambers: CUV-ALL
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