Ocean Optics S2000 - Manual (Page 55)

CUV-UV, CUV-VIS Cuvette Holders
The CUV-UV and CUV-VIS C UVETTE H OLDERS for 1-cm path length cuvettes couple via SMA-terminated optical
fibers to our spectrometers and light sources to create small-footprint spectrophotometric systems for absorbance
and transmission experiments. These holders can be optimized for UV-VIS-NIR (~200-1100 nm) or VIS-NIR
(~360-1100 nm) applications.
Parts Included
" Cuvette holder assembly for holding 1-cm cuvettes
" Black cover for eliminating ambient light
" Two barbed fittings for connecting a temperature stabilizing water source
" Screwdriver for adjusting the fit of the cuvette
" Allen wrench for adjusting the collimating lenses
Operation
Attaching the Fibers
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses. Attach the other end of this
fiber – the illumination fiber – to a light source.
2. Attach another SMA-terminated optical fiber to the other collimating lens. Attach the other end of this fiber –
the read fiber – to the spectrometer.
Using the Fiber Supports
1. Snap the clamps around the fibers after the fibers are screwed into the light source and spectrometer.
2. Lift the clamps until they support the fibers.
3. Unsnap the clamps to remove the fibers.
Installing Filters
1. Loosen the filter clamping screw with the provided screwdriver.
2. Insert the filter into the filter slot. The filter slot can accommodate filters up to 6 mm thick.
3. Clamp the filter in place by gently tightening the clamping screw finger-tight.
Adjusting the Fit of the Cuvette
The Cuvette Holder is designed to hold 1-cm square cuvettes. When properly adjusted, the cuvette should fit snugly
into the holder.
1.
Locate the two ball plunger screws.
2. Remove the fiber clamps for easier access to the screws.
3. Use the provided screwdriver to loosen the two ball plunger screws until the ball end of the screws is just
visible in the cell holder and insert your cuvette into the holder.
4. Tighten the ball plunger screws until the ball contacts the cuvette and starts to compress. Do not over-tighten.
Using the Temperature Stabilization Feature
This feature is used to heat or cool the cuvette holder base and cuvette.
1.
Remove the plugs from the top side of the base. (The plug on the right side of the base should stay in place but
may require thread tape.)
2. Replace the plugs with the two barbed fittings (or any 1/8" NPT pipe thread fittings). Thread tape may be
required on the fittings.
3. Connect the fittings to a water source. Water will circulate through the base.
Sampling Chambers: CUV-UV, CUV-VIS
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