Ocean Optics S2000 - Manual (Page 71)

Assessing if the LPC is Free of Particles
Fluids need to be relatively particle-free. Particles larger than 20 µ m can be trapped inside the tubing and can then
block or scatter a significant amount of light. To rid the LPC of particles, follow these steps:
1.
Pump the sample fluid through the LPC.
2. While in Scope Mode, save a dark spectrum with the light source off and a reference spectrum with the light
source on.
3. Continue to pump the sample fluid and switch to the Absorbance Mode. Ideally, you should see a spectrally
flat line. Particle effects manifest themselves as an exponentially decreasing curve from shorter to longer
wavelengths. The length of time that you pump the sample and the magnitude of the absorbance peak, depends
upon the time required and the minimum detectable absorbance value for your specific analysis. Pre-filtering
of the sample may be required to eliminate this exponentially decreasing absorbance spectrum if it is
significant to your analysis.
Specifications
Sampling Chambers: LPC
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Path lengths:
1-meter, 5-meter and 10-meter options (standard); custom
lengths also available
Tubing:
Teflon  Amorphous Fluoropolymer 2400
~560 µ m inner diameter, ~800 µ m outer diameter
Refractive index: 1.29
Internal volume: 250 µ l/meter
Chemical resistance:
tubing can be altered by perfluorinated solvents, FREON  113,
and Perclene 
Recommended optical fibers for coupling to
spectrometers, light sources: 400 µ m illumination fiber (UV-VIS)
200
µ
m or 400
µ
m read fiber (UV-VIS)
Plumbing fittings: standard ¼" x 28 chromatography fittings