Metrohm 844 UV/VIS Compact IC - Manual (Page 64)

Table of contents
starts up. If column heating has been selected on the tab
Configuration (see Section 3.3.1) then the column heating will
also start up. The chromatogram window of the set data
recorder opens and the baseline(s) are shown continuously.
4 Check for leaks
• Check all the capillaries and their connections from the high-
pressure pump to the detector block for escaping liquid. If
eluent is escaping then the corresponding compression
fitting should be tightened or a new connection should be
made.
5 Condition system
• Rinse the system with eluent until the desired stability of the
baseline is reached (normally 30…60 min; if the eluent is
changed, the establishment of the ion exchanger equilibrium
on the separating column can take longer).
• The instrument is now ready for sample determinations using
the selected system.
2.10.2 Putting into operation with post column reactor
The two Versions IC 2.844.0020 and 2.844.0120 are supplied with post
column reactor
101. Before sample injection can be done, the whole
system must be checked for leaks and conditioned with eluent until a
stable baseline has been achieved. Proceed as follows:
When inserting the column always make sure that it is inserted
correctly in the flow direction shown on the sticker (the arrow must
point in the flow direction).
1 Open and connect system
• Start the «IC Net» PC program, if it has not already been
started.
• Select
File / Open / System in the main window. Open the
system file created in Section 2.5.4 for the 844 UV/VIS
Compact IC.