Metrohm 844 UV/VIS Compact IC - Manual (Page 43)

2.6 High-pressure pump
844 UV/VIS Compact IC / Gebrauchsanweisung 8.844.1051
31
2.6.3 Connecting the eluent bottle
The eluent supply line from the storage bottle to the high-pressure
pump is connected as follows:
Only degassed (with N
2
, He or vacuum) and microfiltered (0.45 μ m
filter) eluents should be used!
The 6.1608.070 Eluent bottle (2 L) supplied is not suitable for vacu-
um degassing. Use a pressure-resistant container for this.
Care must be taken that the eluent used is freely miscible with any
solvent remaining in the pump head (the pump head leaves the
factory filled with either isopropanol or methanol/water). If this is not
the case then the pump must first be rinsed with a solvent which is
miscible with both the previous eluent and the following eluent (e.g.
acetone).
1 Prepare eluent bottle
• Prepare, microfilter (0.45 μm microfilter) and degas (with N
2
,
He, or vacuum) the suitable eluent for the required
application and separating column.
• Fill eluent into eluent vessel 86(clear glass, 2 L).
2 Install bottle attachment
• Firmly screw threaded stopper 84 (6.1446.040; part of
6.1602.160) into the smaller threaded opening (M6) of bottle
attachment 85 (6.1602.105; part of 6.1602.160).
• Firmly screw aspirating filter 87 onto aspirating tubing 82.
• Pull the other end of aspirating tubing 82. through the larger
threaded opening (M8) of bottle attachment 85 from below.
• Push O-ring (E.301.0021; part of 6.1602.160) over the free
end of aspirating tubing 82. and move it towards bottle
attachment 85.
• Push tubing nipple 83 (4.420.4300; part of 6.1602.160) over
the free end of aspirating tubing 82., move it as far as requi-
red towards bottle attachment 85 and screw it loosely in the
larger opening of bottle attachment 85.
• Insert aspirating tubing 82. with screwed-on aspirating filter
87 into eluent bottle 86 and screw bottle attachment 85 onto
eluent bottle 86.
• Pull aspirating tubing 82. so far through the opening of tubing
nipple 83 that aspirating filter 87 is touching the bottom of
eluent bottle 86.
• Fix aspirating tubing 82. in place by screwing shut tubing
nipple 83.