Metrohm 844 UV/VIS Compact IC - Manual (Page 58)

Table of contents
3 Insert separating column
• Push column 113 into the holding clips and fasten column
inlet capillary 47/110 to the inlet of the column (bottom) using
a compression fitting 64 6.2744.010.
• with 2.844.0110 without post column reactor:
• Attach capillary 122 (to the detector) to the outlet of the
column (top) with a compression fitting 64 6.2744.010.
• This will be attached to the detector later (see Section 2.9)
• with 2.844.0120 with post column reactor:
• Attach capillary 123 (to the post column reactor) to the
outlet of the column (top) with a compression fitting 64
6.2744.010.
• This will be attached to inlet "eluent in" 104 of the post
column reactor later (see below, step 6).
4 Close column heating
• Refasten cover 117 onto the column heating using the two
knurled screws 116.
5 Rinse column
• Place a beaker under the eluent flow outlet.
• Open the software window for manual operation.
• If necessary, set the
Flow rate to a suitable value for the
selected column and send it to the 844 UV/VIS Compact IC
with .
• Switch on the high-pressure pump (
IC pump
) by clicking on
and rinse the column with eluent for approx. 10 min.
• Switch off the high-pressure pump by clicking on .
6 Connect column to post column reactor
Only with Version 2.844.0120 (with Version 2.844.0110 please go
directly to Section 2.8.4.3):
• Attach capillary 123 to inlet "eluent in" 104 of the post column
reactor with a compression fitting 64 6.2744.010.