General information on separating columns – Metrohm 844 UV/VIS Compact IC User Manual
Page 52
Table of contents
New IC precolumns are normally filled with solution and sealed at both
ends. Before the precolumn is installed in the system, it must be
ensured that this solution is freely miscible with the eluent used
(check manufacturer's specifications).
1 Connect precolumn
• The precolumn is mounted upstream from the separating
column. Use as short a piece of 6.1831.010 PEEK capillary as
possible.
• If column heating is used (see Section 2.8.4 the precolumn
can also be placed in the inner compartment of the column
heating. Use an S-Guard for this. Other precolumns can only
be mounted outside the column heating.
When you install the column, always ensure that this is inserted
correctly in accordance with the flow direction (if existent) shown.
2 Rinse the precolumn
• Place a beaker beneath outlet capillary.
• Switch on IC Pump in «IC Net» and rinse precolumn for
approx. 10 min with eluent.
• Switch off IC Pump.
2.8.2 General
information
on separating columns
The precolumn that is suitable for your separating column can be
found in the
Metrohm IC Column Catalog, the product information
about separating columns that can be found under
on chromatography products, or let your agency advise
you directly.
New IC separating columns are normally filled with solution and
sealed at both ends. Before the column is installed in the system, it
must be ensured that this solution is freely miscible with the eluent
used (check manufacturer's specifications).
The separating columns currently available from Metrohm and preco-
lumns can be found in the Metrohm IC Column Catalog, or on the
Internet under
in the ion chromatography
product section. Each column is supplied with a test chromatogram
and a data sheet. More detailed information about special IC
applications can be found in the relevant "Application Bulletins" or
"Application Notes"; these are available on the Internet under
applications sector, or can be
requested free of charge from your local Metrohm agency.
When you install the column always ensure that this is inserted
correctly in accordance with the flow direction shown.