Eppendorf BioSpectrometer kinetic - Manual (Page 94)

Evaluation procedure
Eppendorf BioSpectrometer
® kinetic
English (EN)
94
12.5.3 Conversion to molar concentrations and nucleic acid quantities
The conversion only can be applied to nucleic acids and dye methods with nucleic acids as biomolecule
components. It is realized in the
process results/More calculations method step.
12.5.3.1 Calculation of amount
Application: calculating the amount (mass) of nucleic acid in the total sample volume.
M = calculated total amount (mass) of nucleic acid in the sample tube. Unit: μg.
C = nucleic acid concentration calculated from the measurement. Unit: μg/mL or ng/μL.
V
S, total
= total volume of the sample in the sample tube. Enter this value in More calculations. Unit: μL.
12.5.3.2 Calculation of the molar concentration
Application: calculation of the molar concentration of the nucleic acid from the mass concentration and
relative molar mass. The molar mass is either entered directly or calculated by the device from the entered
number of bases or base pairs per nucleic acid molecule.
C
Mol
= calculated molar concentration of the nucleic acid. Unit: pmol/mL.
C = nucleic acid concentration calculated from the measurement. Unit: μg/mL or ng/μL.
MM = relative molar mass. Unit: kDa
If the number of bases or base pairs per nucleic acid molecule are entered in More calculations instead of
the relative molar mass, the MM is calculated from the number of the bases or base pairs:
For dsDNA:
For ssDNA, RNA, Oligo:
MM = calculated relative molar mass; unit: kDa
bp = entered number of base pairs per molecule
b = entered number of bases per molecule
MM
C
C
Mol
3 10
u