Eppendorf BioSpectrometer kinetic - Manual (Page 88)

Technical data
Eppendorf BioSpectrometer
® kinetic
English (EN)
88
11.7 Application parameters
Methods Preprogrammed and freely programmable methods for all measuring
and evaluation procedures:
• Absorbance measurements with one or more wavelengths, scans
• Nucleic acids and proteins, OD600, dye methods (parallel
measurement of biomolecule and dye marking)
• Methods with evaluation via factor, standard and standard series
• Dual wavelength procedure with subtraction and division
evaluation
• Kinetic method: End point, two-point, linear regression
Method-dependent evaluation Absorbance, concentration via factor and standard.
Concentration via standard series:
• Linear regression
• Nonlinear regression (2nd and 3rd degree polynoms)
• Spline evaluation
• Linear interpolation (point-to-point evaluation)
Absorbance calculations via subtraction and division
Additional data for nucleic acids: Ratios 260/280 and 260/230; molar
concentration, total yield
Additional data for dye methods: FOI (frequency of incorporation,
labeling density)
Scans: zoom, peak evaluation
Kinetics: Subsequent modification of the time window for the
regression evaluation
Method memory >100 method programs
Measured value memory and
calibration memory Memory for >1 000 results with all data of the results evaluation and
standard evaluation, sample number, sample name, date and used
parameter set of the method program.
(The number of saved results depends on the number of saved
methods.)