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Eppendorf BioSpectrometer kinetic User Manual

Page 34

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Methods
Eppendorf BioSpectrometer

®

kinetic

English (EN)

34

Unit

Selection:
mg/mL | μg/mL | 
ng/mL | pg/mL | μg/μL
| mg/dL | μmol/mL |
nmol/mL | pmol/mL |
pmol/μL | U | U/mL |
U/L | % | Abs | A/min
In addition, further
units are freely
programmable in the
General Method
Parameters/Units
function. Max. 7 digits.

Unit for the concentration result.
In the preprogrammed methods of the Routine group, the
selection is restricted to units that are useful for these methods.

Formula type

Selection:
division | subtraction

Only for the

Dual wavelength method group.

Formula type for calculating the absorbances at the two
measurement wavelengths prior to evaluation with factor or
standard.

Formula: a

Value input:
Value for a in the
evaluation formula.
Limit: max. of 5 digits
including decimal
point.

Only for the

Dual wavelength method group.

Value for a in the formulas: 
[(a*A1) / (b*A2)] * c + d and [(a*A1) - (b*A2)] * c + d.

Formula: b

Value input:
Value for b in the
evaluation formula.
Limit: max. of 5 digits
including decimal
point.

Only for the

Dual wavelength method group.

Value for b in the formulas: 
[(a*A1) / (b*A2)] * c + d and [(a*A1) - (b*A2)] * c + d.

Formula: c

Value input:
Value for c in the
evaluation formula.
Limit: max. of 5 digits
including decimal
point.

Only for the

Dual wavelength method group.

Value for c in the formulas: 
[(a*A1) / (b*A2)] * c + d and [(a*A1) - (b*A2)] * c + d.

Formula: d

Value input:
Value for d in the
evaluation formula.
Limit: max. of 5 digits
including decimal
point.

Only for the

Dual wavelength method group.

Value for d in the formulas: 
[(a*A1) / (b*A2)] * c + d and [(a*A1) - (b*A2)] * c + d.

Calculation

Selection:
factor | standard

Evaluation procedure for the calculation of the sample
concentration from the measured absorbance.

Parameter

Entry

Explanation