Eppendorf BioSpectrometer kinetic - Manual (Page 36)

Methods
Eppendorf BioSpectrometer
® kinetic
English (EN)
36
Correct A260 1 Selection:
on | off Only for the Dye labels method group.
Correction of the influence of the dye spectrum on the
absorbance with the measuring wavelength of the biomolecule
(260 nm or 280 nm). Some of the dye spectra have a low
absorbance at 260 and 280 nm. These absorbances distort the
calculations for the nucleic acids or the proteins of these
methods. To minimize the distortion correction factors are
used, as long as these are known for the corresponding dyes.
When the parameter is switched on, the correction factor is
imported from the
General Method Parameters/Dyes function.
Correct A 280 1 Selection:
on | off Only for the Dye labels method group.
For an explanation see the description of the Correct A260 1
parameter above.
Dye 2 active Selection:
on | off Only for the Dye labels method group.
Option to measure a second dye at the same time. Application:
Labeling a biomolecule with two dyes.
Dye 2 Selection:
List of dyes that are
stored in the
General
Method Parameters/
Dyes function.
Only for the Dye labels method group when measuring 2 dyes.
Selection of the second dye (see Dye 1 parameter).
Correct A260 2 Selection:
on | off Only for the Dye labels method group when measuring 2 dyes.
Analog to parameter Correct A260 1.
Correct A280 2 Selection:
on | off Only for the Dye labels method group when measuring 2 dyes.
Analog to parameter Correct A280 1.
Show scan Selection:
on | off Display of a scan (absorbance wavelength graph) in addition to
the result of the sample measurement.
Start λ Value input:
Wavelength in nm.
Range: 200 to 830 nm. Starting wavelength for recording the scan.
Stop λ Value input:
Wavelength in nm.
Range: 200 to 830 nm.
Value must be higher
than the value for 
Start λ. Stopping wavelength for recording the scan.
A260/A280 Selection:
on | off Only for nucleic acids.
Display of the A260/A280 ratio in addition to the result of the
sample measurement.
A260/A230 Selection:
on | off Only for nucleic acids.
Display of the A260/A230 ratio in addition to the result of the
sample measurement.
Parameter Entry Explanation