Eppendorf BioSpectrometer kinetic - Manual (Page 33)

33
Methods
Eppendorf BioSpectrometer ® kinetic
English (EN)
6.2.4 Advanced method group
Dual wavelength
• Measurement at two wavelengths and evaluation of the measured absorbance values via two basic
formulas (subtraction, division)
• Variants of the basic formulas can be defined.
• The result can be evaluated with a factor, with a standard or with a standard series.
• Methods are preprogrammed for calculation, subtraction and division, and subsequent factor
evaluation.
Advanced kinetics
The following options are available via the description of the Simple kinetics method (Basic method group):
• Measurement of a reagent blanks kinetics. The blank result is subtracted from all sample results prior to
the evaluation.
• An alternative to evaluation via factor is evaluation via a standard.
6.3 Method parameters
This chapter illustrates the parameters for programming the methods. The order of the parameters in the
device display may slightly differ from the order in the table in order to display the parameters more clearly.
The table displays all parameters available for the various methods. Only a small portion of these
parameters are required for the corresponding method and will be shown in the display.
Parameter Entry Explanation
Cuvette Selection:
10 | 5 | 2 | 1 | 0.5 | 0.2 |
0.1 mm Optical path length of the cuvette. The device always
automatically converts absorbance values to the 10 mm path
length of a standard cuvette (see Absorbance values on p. 89).
Therefore, there is no need to change factors such as "50" for
the calculation of dsDNA concentrations when modifying the
Cuvette parameter.
No. of
wavelengths Value input:
Range: 2 to 6. Only for the Multi λ method group.
Number of wavelengths at which the measurement is to be
performed.
Wavelength Value input:
Measurement
wavelength in nm.
Range: 200 to 830 nm. Measurement wavelength: The concentration is calculated
based on the absorbance measured with this wavelength.
For the
Multi λ and Dual wavelength method groups, enter
more than one wavelength. For some method groups (e.g.,
Nucleic acids and Proteins direct UV), the wavelengths are
preprogrammed.
For the
Dye labels method group the measuring wavelengths
are not entered into the method procedure one by one. They
are automatically imported simply by selecting the biomolecule
and dye from the
General Method Parameters function.