Eppendorf BioSpectrometer kinetic - Manual (Page 27)

27
Operation
Eppendorf BioSpectrometer ® kinetic
English (EN)
Measured values typical for Eppendorf for temperature control in closed cuvettes, with closed cuvette shaft
covers, are shown in the following tables. The temperature was measured in the measuring solution; the
ambient temperature was 24.5°C.
Tab. 5-1: The temperature to be set for temperature control of a measuring solution
Tab. 5-2: Duration for temperature control of a measuring solution
Cuvette type/measuring volume Target temperature Temperature to be set in the
method parameters
Quartz glass macrocuvette, 
1 500 μL 25°C 25°C
30°C 30.4°C
37°C 37.7°C
Plastic macrocuvette, 
1 000 μL 25°C 25°C
30°C 30.4°C
37°C 37.7°C
Quartz glass semimicrocuvette,
500 μL 25°C 25°C
30°C 30.4°C
37°C 37.7°C
Quartz glass ultra-micro cuvette,
60 μL 25°C 25°C
30°C 30.3 °C
37°C 37.6°C
Cuvette type/measuring volume Initial temperature and target
temperature Temperature control duration
Quartz glass macrocuvette, 
1 500 μL 25°C - 37°C Approx. 7 min
37°C - 25°C Approx. 11 min
25°C - 30°C Approx. 7 min
Plastic macrocuvette, 
1 000 μL 25°C - 37°C Approx. 13 min
37°C - 25°C Approx. 19 min
Quartz glass semimicrocuvette,
500 μL 25°C - 37°C Approx. 7 min
37°C - 25°C Approx. 12 min
Quartz glass ultra-micro cuvette,
60 μL 25°C - 37°C Approx. 7 min
37°C - 25°C Approx. 9 min
25°C - 30°C Approx. 5 min