Eppendorf BioSpectrometer kinetic - Manual (Page 80)

Troubleshooting
Eppendorf BioSpectrometer
® kinetic
English (EN)
80
9.3 Result flags
Warnings and error messages for results are displayed in the bottom right of the help box. The header bar
of the Help box is highlighted yellow for warnings and red for error messages.
Warnings: Decide whether the result is useful for you while taking the displayed warning into
consideration.
Error messages: No result is displayed; the reason is shown in the error message.
Problem Cause Solution
The standard curve
is not monotone.
Please select
another Curve Fit. • No usable result was returned during the
evaluation of a standard curve using the
"spline interpolation", "quadratic
regression" or "cubic regression"
Curve
Fit procedures.
 Select a different Curve Fit
procedure.
Some absorbance
values for
secondary
wavelengths are too
high or are not
displayed. • For at least one secondary wavelength,
the absorbance exceeded the measuring
range.
• Secondary wavelengths are not needed
for calculating the concentration result.
They are used for different purposes. For
example,
dsDNA method: absorbance at
280 nm for the calculation of ratios 260/
280.
• Turbidity of the measuring solution
• Measurements at the limits of the
photometric measuring range.
 If the absorbance values of the
secondary wavelengths are
relevant: Dilute the sample or
remove the turbidity via
centrifugation and repeat the
measurement.
The result is
outside the range of
the standard
concentrations. • For methods with evaluation via standard
curves (nonlinear evaluation method):
The sample result is up to 5 % outside of
the standard concentration range.
 Accept the measurement result, or
remeasure the sample under
conditions under which the result
is within the range of the standard
concentrations (dilute sample or
modify standard concentrations
and remeasure).
The coefficient of
determination is
<0.8. • For methods with evaluation of standard
series via the regression procedure: The
coefficient of determination for the
regression evaluation indicates a
significant deviation of the measuring
points from the regression line.
• Turbidity of the measuring solution.
• Measurements at the limits of the
photometric measuring range.
 Accept the result of the standard
evaluation or remeasure the
standards.
 Make sure the measuring
solutions are clear.