Eppendorf BioSpectrometer kinetic - Manual (Page 91)

91
Evaluation procedure
Eppendorf BioSpectrometer ® kinetic
English (EN)
The factor is either entered directly as a parameter during the "Factor" evaluation procedure or calculated
during the "Standard" evaluation procedure (evaluation with a standard concentration):
F = calculated factor.
C
S
= concentration of the standard (enter as parameter).
A
S
= measured absorbance of the standard.
If multiple measurement (2 or 3 replicates) has been programmed for the standard, the average value is
calculated from the measured absorbance values and inserted as A
S
.
12.3 Evaluation with standard curve/line
If evaluations are made with more than one standard, the following evaluation procedures for the standard
curve/line can be selected with the [Curve fit] in the
measure standards/new method step:
For the regression procedure, one can select that the regression line (regression curve) goes through the
zero point.
Evaluation procedure Description Minimum required number of
standard points
Linear interpolation Linear point-to-point connection in the
absorbance concentration graph of the
standard evaluation. 2 standards minimum.
Linear regression Polynome regression for first degree
polynomial. 3 standards minimum.
Quadratical regression Polynome regression for second degree
polynomial. 4 standards minimum.
Cubical regression Polynome regression for third degree
polynomial. 5 standards minimum.
Spline interpolation Interpolation via natural cubic splines. 3 standards minimum.
S
S
A
C
F