Eppendorf BioSpectrometer kinetic - Manual (Page 35)

35
Methods
Eppendorf BioSpectrometer ® kinetic
English (EN)
Factor Value input:
Factor.
Limit: max. of 6 digits
including decimal
point. Factor for converting absorbance values into the concentration.
Negative factors can also be entered for the following method
groups:
Simple kinetics, Advanced kinetics, Dual
wavelength, Factor.
For the
Dye labels method group the factors are not entered
into the method procedure individually. They are automatically
imported simply by selecting the biomolecule and dye from the
General Method Parameters function.
Protein Selection:
List of protein types
which are stored in the
General Method
Parameters/Proteins
function. Only for the Dye labels and Proteins direct UV method
groups.
When selecting the protein, the corresponding
Factor
parameter programmed in the General Method Parameters/
Proteins function also will be imported from that function.
Standards Value input:
Number of standards.
Range: 1 to 12. Number of different standard concentrations for the evaluation
with standards.
For some methods the range for the number of standards is
restricted to a smaller range than 1 to 12.
Replicates Value input:
Number of replicates
per standard.
Range: 1 to 3. Number of repeated measurements for the various standard
concentrations.
Std. conc. Value input:
Concentration values
of the standards.
Limit: max. of 6 digits
including decimal
point. Based on the number of standards, this parameter is available
for all standards (e.g.,: std. conc. 1, std. conc. 2, ...).
Decimal places Value input:
Number of decimal
points for the result.
Range: 0 to 3. Number of decimal points for the calculated concentration
result.
Dye 1 Selection:
List of dyes that are
stored in the
General
Method Parameters/
Dyes function.
Only for the Dye labels method group.
When the dye is selected, the parameters programmed in the
General Method Parameters/Dyes function, which correspond
to the dye, are also imported: factor, wavelength and, if
necessary, correction factors for the measurement at 260 or
280 nm (see the description of the following parameter).
Parameter Entry Explanation