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4 photometric properties, 5 incubation, Photometric properties – Eppendorf BioSpectrometer kinetic User Manual

Page 86: Incubation, 4 photometric properties 11.5 incubation

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Technical data
Eppendorf BioSpectrometer

®

kinetic

English (EN)

86

11.4

Photometric properties

11.5

Incubation

Measuring principle

Single beam absorption spectrophotometer with reference beam

Light source

Xenon flash lamp

Monochromator

Holographic aberration-corrected concave grating

Beam receiver

CMOS photodiode array

Wavelengths

200 nm to 830 nm

Wavelength selection

Method-dependent, freely selectable

Spectral bandwidth

≤ 4 nm

Smallest step size

1 nm

Systematic wavelength error

±1 nm

Random wavelength error

≤ 0.5 nm

Photometric measuring range

0 A to 3.0 A at 260 nm

Reading accuracy

ΔA = 0.001

Random photometric error

≤ 0.002 at A = 0
≤ 0.005 (0.5 %) at A = 1

Systematic photometric error

±1 % at A = 1

Stray light component

< 0.05 %

Adjustable temperature range

20°C to 42°C

Smallest step size

0.1°C

Maximum temperature
uncertainty (in relation to the
temperature in the sample)

(see Tab. on p. 27)

Systematic temperature error

±0.2 °C at 25 °C to 37 °C

Random temperature error

±0.15 °C at 25 °C to 37 °C