Eppendorf BioSpectrometer kinetic - Manual (Page 37)

37
Methods
Eppendorf BioSpectrometer ® kinetic
English (EN)
FOI Selection:
none | dye/kb | pmole/
μg Only for the Dye labels method group.
Display of the FOI in addition to the result of the sample
measurement.
The FOI (frequency of incorporation) is a measure for the
number of dye molecules per nucleic acid molecule that are
integrated into the nucleic acid. Units are "dye/kb" (dye
molecules per 1000 bases) or "pmole/μg" (pmol dye per μg
nucleic acid). "None": no FOI calculation.
Background Selection:
on | off Prior to the calculation of the results of a sample the
absorbance of a background wavelength, during which the
analyte to be measured should exhibit the absorbance value
zero, is subtracted from the absorbance of the measuring
wavelength. Frequent application: Partial correction of
turbidity for measurement of nucleic acids (background
wavelength in this case: 320 nm or 340 nm).
Wavelength Wavelength in nm.
Range: 200 to 830 nm. Wavelength at which the background is to be measured. The
analyte to be measured should have the absorbance value zero
in pure form here.
Background for
dyes Selection:
on | off Only for the Dye labels method group.
Application of the background correction to the measurement
of a dye (see
Background parameter).
Wavelength Wavelength in nm.
Range: 200 to 830 nm. Only for the Dye labels method group.
Wavelength at which the background for the dye is to be
measured. The pure, not contaminated dye to be measured
should have the absorbance value zero for this wavelength.
Temperature on Selection:
on | off
For kinetic methods only.
Use of cuvette temperature control.
Temperature Value input:
Temperature in °C.
Range: 20 to 42°C. For kinetic methods only.
Temperature input for the cuvette temperature control when
the
Temperature parameter is set to "on".
Notice! Damages and measurement errors due to
condensate.
At high air humidity, condensate may form on a cuvette having
a significantly lower temperature than the ambient
temperature. The condensate may cause damage to the optics
and lead to incorrect measuring results.
 The temperature of the cuvette should not remain
significantly below the ambient temperature for a longer
period of time. If required, observe the actual dew point.
Parameter Entry Explanation