Eppendorf BioSpectrometer kinetic - Manual (Page 82)

Troubleshooting
Eppendorf BioSpectrometer
® kinetic
English (EN)
82
During the kinetics
measurement, the
temperature was
outside of the
allowable range. • Ambient temperature outside the
specified range.
• The temperature control is faulty.
 Measure the sample at ambient
temperature within the specified
range (15°C to 35°C). If the
warning nevertheless appears,
contact Eppendorf Service.
Absorbance at the
measuring
wavelength is too
high. • Turbidity of the measuring solution.
• Optical surfaces of the cuvette are soiled.
• Cuvette has been inserted into the
cuvette shaft facing the wrong direction.
• Too high absorbance of measuring
solution.
 Measure again considering the
possible causes.
The calculated
result is negative. • Measuring solution not prepared
correctly.
• The incorrect factor has been entered
(wrong algebraic sign).
 Measure again considering the
possible causes.
At least one of the
results is negative. • For methods with several results (e.g.,
Dye labels).
• Measuring solution not prepared
correctly.
• The incorrect factor has been entered
(wrong algebraic sign).
 Measure again considering the
possible causes.
The result has more
than 6 pre-decimal
places. • Very high sample concentration.
• Concentration unit does not match the
expected range of the sample
concentrations.
 Dilute sample and measure again.
 Change the concentration unit
(Parameter Unit) and measure
again.
The result is more
than 5 % outside of
the standard
concentration
range. • For methods with evaluation via standard
curves (nonlinear evaluation method):
The sample result is more than 5 %
outside of the standard concentration
range.
 Remeasure the sample under
conditions under which the result
is within the range of the standard
concentrations (dilute sample,
modify standard concentrations
and remeasure).
• Calculation not
possible
because of
division by zero.
Absorbance
result is zero.
• Calculation
error. Division
by zero.
• The evaluation required dividing by an
absorbance result with the value of
"zero". This is not mathematically
permissible.
Examples: Calculation of a factor at
one-point calibration; calculation of a
260/280 ratio with nucleic acid
measurements.
 Check the reagents and samples
used and repeat the measurement.
Calculation not
possible because of
division by zero.
Absorbance result
or parameter
formula
b is zero.
• An absorbance result was divided by a
"zero" value during the evaluation of a
Division type method (Dual wavelength
method group). This is not
mathematically permissible.
 Check the reagents and samples
used and repeat the measurement.
 Do not enter "zero" as a value for
the Formula b parameter.
Problem Cause Solution