Eppendorf BioSpectrometer kinetic User Manual
Page 38
Methods
Eppendorf BioSpectrometer
®
kinetic
English (EN)
38
Measuring
procedure
Selection:
lin.regr. | endpoint |
two point
For kinetic methods only.
"Linear regression": Measurement over several periods in set
time intervals within a defined period of time. Evaluation via
linear regression of the absorbance time graph within the
measuring time. Absorbance result: ΔA/min.
"Endpoint": Measurement of a measuring point after a defined
period of time. Absorbance result: A.
"Two point": Measurement of 2 measuring points at defined
times. Evaluation: Via linear interpolation between these
measuring points in the absorbance time graph. Absorbance
result: ΔA/min.
Reagent blank
Selection:
on | off
For kinetic methods of the
Advanced kinetics method group
and evaluation with factor only.
Measurement of a reagent blank (RB). The RB is measured
according to the same measurement method as a sample. The
absorbance result in A or ΔA/min is subtracted from the
absorbance result of the sample before the sample
concentration is calculated. Application: Correction of sample
results for kinetics with reagent drift. The reagent blank
contains the reagent and demineralized water as sample.
Delay
Value input:
Time from the start to
the first measuring
point.
Range: 00:00 to 10:00
min:sec.
For kinetic methods only.
Time from the start of the measuring procedure to recording
the same measuring point.
Measuring time Value input:
Time between first and
last measuring point.
Range: 00:05 to 59:59
min:sec.
Only for kinetic methods and the "lin.regr." and "two point"
measurement methods.
Time between recording the first and the last measuring point.
Total time
Value input:
Time between first and
last measuring point.
Range: 00:05 to 59:59
min:sec.
For the
Single λ - cont kinetic method only.
Time between recording the first and the last measuring point.
Interval
Value input:
Time between two
measuring points.
Range: 00:05 to 10:00
min:sec.
Only for kinetic methods and "lin.regr." measurement methods.
Time intervals between the measuring points.
Autoprint
Selection:
on | off
Printing a measuring result immediately following
measurement with the thermal printer.
Only the main result data will be printed. To output detailed
data, the required data packets can be compiled and printed in
the
print & export method step at the end of a measuring
series.
Parameter
Entry
Explanation