Eppendorf BioSpectrometer kinetic - Manual (Page 47)

47
Methods
Eppendorf BioSpectrometer ® kinetic
English (EN)
Proteins
direct UV Results display:
• Concentration result with absorbance
at the measuring wavelength
• If activated in the parameters: Scan.
Navigate between the measuring
points on the graph which are used for
the result calculation with and .
Additional data ([Data] softkey):
If the corresponding parameters have
been activated:
• Absorbance value for 260 nm.
• Absorbance value for the background
wavelength.
Proteins (with
reagent) Results display:
• Concentration result with absorbance
at the measuring wavelength.
• For evaluation with standard series:
graph of the standard evaluation with
plotted sample result.
Dye labels Results display:
• Concentration results with absorbance
at the measuring wavelength of the
biomolecule.
• If activated in the parameters: Scan.
Navigate between the measuring
points in the graph with and .
Additional data ([Data] softkey):.
If the corresponding parameters have
been activated:
• Ratios A260/A280 and A260/A230.
• Absorbance values for 280 nm and
230 nm and for the measuring
wavelength of the dye.
• FOI value.
• Absorbance values for the background
wavelengths.
Ratios and FOI are not displayed for the
measurement of the dye-labeled proteins.
Method group Results display Explanation