Eppendorf BioSpectrometer kinetic - Manual (Page 67)

67
Maintenance
Eppendorf BioSpectrometer ® kinetic
English (EN)
8.3.1 Checking the spectrometer unit
Eppendorf offers a filter kit (BioSpectrometer reference filter kit) for checking the photometric accuracy
and wavelength systematic error. The kit contains one blank filter (A0) and three filters (A1, A2 and A3) for
checking the photometric accuracy, and 3 filters for checking the wavelength systematic error in the range
of 260 nm to 800 nm. The filter absorbances are measured against blank filter A0. In addition to
information on accuracy, you also obtain information on precision: average value and variation coefficient
(CV value) are calculated from the sets of 15 measurements per wavelength.
For the measurement, place the blank filters (for blank measurement) and sample filters into the cuvette
shaft, using the same procedure as with cuvettes. The absorbance values measured for the test filters will
be compared to the permitted range of values. For the individual filters, the limit values for the permitted
area are printed in a table in the lid of the filter box.
Please connect the Eppendorf thermal printer if you would like to document the values.