Eppendorf BioSpectrometer kinetic - Manual (Page 22)

Operation
Eppendorf BioSpectrometer
® kinetic
English (EN)
22
Prerequisites
• The cuvette is free from contamination by dust or fingerprints and free from scratches.
• The cuvette shaft is free from particles, dust and liquid.
• The measuring volume in the cuvette is sufficient. Ensure that the minimum measuring volume has
been reached.
• The measuring solution is free from particles and bubbles.
1. Position the cuvette so that the optical window of the cuvette is pointing towards the direction of the
light path.
2. When inserting the cuvette, press it completely to the bottom against the slight resistance.
5.3 Summary of the measuring procedure
5.3.1 Preparing the measurement
1. Switch on the device and, if required, the printer.
The device performs a self test (taking approx. 1 minute) and displays the method selection.
2. Make ready the cuvettes for the measurements (see Inserting the cuvette on p. 21).
3. Prepare the measuring solutions for measuring the blank values, if required, also the standards and the
samples.
4. Open the cover of the cuvette shaft. The cover can remain open during the measurements.
The direction of the light path is marked with an arrow on the housing.
You should not use any measuring solution for standards and samples with a lower
absorbance than 0.02 to 0.03 A (e.g. dsDNA concentration between 1.0 and 1.5 μg/mL). The
detection limit of the device may be significantly lower, nevertheless, the impact of
disturbances from the measuring solutions (particles, bubbles, turbidity) on the reliability of
the result is very high for these low absorbance values.
Cuvettes
* or similar microliter cuvette
See manufacturer
information
See manufacturer
information
Macro Semi-micro Ultra-micro UVette ® Hellma ® TrayCell *
50 µL 70 µL 400 µL 1000 µL
Eppendorf
µCuvette G1.0
Basic area 12.5 mm × 12.5 mm
Min. overall height 36 mm
Min. filling level 10 mm
Light path 8.5 mm
Max. height of base 7 mm
0 mm
Min. volume Photometry