Eppendorf BioSpectrometer kinetic User Manual

Page 62

Functions
Eppendorf BioSpectrometer

kinetic

English (EN)

When programming a method of the

Dye labels or Proteins direct UV method groups, you can access the

entries in

General Method Parameter:

Tab. 7-2:

Parameter in General Method Parameter

Select the name of the dye to import the
corresponding parameter group into the method
program. By using the "edit" selection of the
"Nucleic acid" parameter, you also can get directly
to the

General Method Parameter function and

view and edit the parameters.

Parameter

Explanation

Proteins

These parameters are loaded into the method parameters when a protein is
selected during the programming of a method in the

Dye labels and Proteins

direct UV groups.

• Protein name
• Factor
• A

0.1%

• Ext.coeff.
• Molecular mass

In order to define a factor for calculating the concentration on the basis of the
absorbance, enter the following data in addition to the name and wavelength:
Factor

or A

0.1%

or absorbance coefficient and molar mass.

Nucleic acids

These parameters are loaded into the method parameters when a nucleic acid
is selected during the programming of a method in the

Dye labels group.

• NA name
• Factor
• Double-stranded

The factor is used to calculate the concentration on the basis of the
absorbance.
The double-stranded parameter affects the calculation of the molar nucleic
acid concentration. (see Conversion to molar concentrations and nucleic acid
quantities on p. 94)

Dyes

These parameters are loaded into the method parameters when a dye is
selected during the programming of a method in the

Dye labels group.

• Dye name
• Wavelength
• Ext.coeff.
• Factor
• Corr. A260
• Corr. A280

In order to define a factor for calculating the concentration on the basis of the
absorbance, enter the following data in addition to the name:
Factor

or absorbance coefficient.

The correction factors for the absorbance values at 260 or 280 nm are used
when the correction function in the method parameters is active. For more
details, refer to the chapter on evaluation (see Correction A

Units

You can select a unit from all available units when programming method
parameters.

• Unit

Entering a unit that has not yet been programmed for the concentration result.