Eppendorf SOP - Manual (Page 9)

9
Calibration
Eppendorf SOP
English (EN)
2.1.4 Bottle-top dispenser and Top Buret
For bottle-top dispensers and the Top Buret, check the nominal volume with 10
measured values.
2.2 Measurement
2.2.1 Work method
1. Place the selected pipette tip on the tip cone of the pipette or on the
corresponding Combitip in the Multipette.
2. Implement the following settings:
• Adjustable-volume piston-stroke pipettes: the smallest volume to be tested
• Multipettes: the nominal volume
• Bottle-top dispenser and Top Buret: the nominal volume
3. For all Multipettes with fully drawn up Combitip, always discard the first
dispensing step.
4. Fill test liquid up to a height of min. 3 mm in the weighing vessel.
5. For piston-stroke pipettes fill the pipette tip 5x with test liquid and empty it
(pre-wet), in order to create a moisture balance in the dead air volume.
6. Replace the single-use tip.
7. Pre-wet the tip 1x.
2.2.2 Removing the test volume from the reservoir
1. Hold the pipette vertically.
2. Dip the pipette tip into the test liquid by a few millimeters.
3. Draw in the volume to be tested slowly and evenly. The waiting time of 1 to
3 seconds, for Research 1 to 10 mL 5 seconds must be observed.
(the waiting time corresponds to the size of the tip, see operating
instructions.)
4. Pull the pipette tip slowly out of the liquid, wiping it on the vessel wall.