Eppendorf SOP - Manual (Page 33)

33
Adjustment
Eppendorf SOP
English (EN)
7.3 Adjusting fixed-volume pipettes
The adjustment process for fixed-volume pipettes is identical to the adjustment
process for variable pipettes (see
Adjusting adjustable-volume pipettes on p. 17).
For fixed-volume pipettes, 10 measured values of the nominal volume are checked.
7.3.1 Reference fixed
Auxiliary equipment
• Adhesive label as an adjusting aid for the basic setting
• Supplied pipette wrench (order no. 4910 092.001)
One revolution of the control button corresponds to the following (based on water):
1. In order to simplify relocating the basic
setting, attach the adhesive label
supplied as an adjusting aid to the
control button.
2. Using the B side of the pipette wrench,
loosen the inside screw until the control
button can be turned.
3. Set the control button to the calculated
actual volume value of the measurement
(see Adjusting adjustable-volume
pipettes on p. 17).
Reference fixed-volume Vol./turn
1, 2, 5, 10 μL approx. 0.5 μL
10, 20 μL approx. 1 μL
25, 50 μL approx. 2.4 μL
100 μL approx. 5 μL
200, 250 μL approx. 12 μL
500, 1 000 μL approx. 46 μL
1 500, 2 000, 2 500 μL approx. 118 μL
0
4
4
0