Eppendorf SOP - Manual (Page 12)

12
Sterilization and cleaning
Eppendorf SOP
English (EN)
4 Sterilization and cleaning
4.1 Sterilization
Parameter for autoclaving
• 121 °C
• 20 minutes
• 1 bar overpressure
4.1.1 Reference and Biomaster
The Reference pipettes and the Biomaster are fully autoclavable.
1. Prior to autoclaving, unscrew the upper and lower part of the pipette apart by
approx. one turn to enable the vapor to enter more easily.
2. After autoclaving, allow the pipette to cool down to room temperature and to
dry completely and then screw together.
4.1.2 Research plus and Reference 2
The Research plus pipettes and Reference 2 pipettes are fully autoclavable.
1. You can put the Research plus and Reference 2 into the autoclave as a
complete unit or with the lower part removed. Do not disassemble the lower
part.
2. For 2.5 mL, 5 mL and 10 mL pipettes: remove the old protection filter. Add a
new protection filter and install it after autoclaving. Only autoclave the
protection filter once.
After autoclaving:
3. Cool the pipette down to room temperature and leave to dry.
4. For 2.5 mL, 5 mL / 10 mL pipettes: the protection filter swells during
autoclaving. Slightly squeeze the protection filter when installing it into the
cone tip.
Further notes on cleaning and sterilization can found in the relevant
operating manual.