Eppendorf SOP - Manual (Page 8)

8
Calibration
Eppendorf SOP
English (EN)
2 Calibration
2.1 Scope of testing
Note concerning the nominal volume:
The nominal volume of an adjustable-volume pipette is the largest volume to be
set by the user and specified by the manufacturer.
When using the Combitip with the Multipette, the nominal volume is the largest
possible dispensing volume of the Combitip. This means that for the Multipette
plus and Multipette M4, the nominal volume is 1/5 of the filling volume of the
Combitip advanced. For the Multipette stream / Xstream, the nominal volume is
the filling volume of the Combitip advanced.
2.1.1 Adjusting adjustable volume pipettes
For variable pipettes, check 3 different volumes with 10 measured values each:
• the nominal volume,
• approx. 50 % of the nominal volume,
• 10 % of the nominal volume.
2.1.2 Multi-channel pipettes
For Multi-channel pipettes check each channel separately with 3 different
volumes, each with 10 measured values:
• the nominal volume,
• approx. 50 % of the nominal volume,
• 10 % of the nominal volume.
2.1.3 Multipette
With the
• Multipette,
• Multipetteplus,
• Multipette stream,
• MultipetteXstream
• MultipetteM4
check the nominal volume with 10 measured values with the Eppendorf
Combitip used.