Eppendorf SOP - Manual (Page 15)

15
Leakage check
Eppendorf SOP
English (EN)
5 Leakage check
5.1 Pipette leakage check
In order to carry out a leakage check on a pipette, follow the procedure outlined
below:
1. For adjustable-volume pipettes: set the nominal volume.
2. For volumes <20 μl pre-wet the tip several times.
3. Hold the pipette vertically with a full tip for approx. 30 sec. Do not touch the
pipette tip.
4. Observe the meniscus of the liquid on the tip opening. If there is a leak in the
pipette, a drip will form on the tip opening.
6 Troubleshooting
6.1 Potential error causes and corrections
Observe the error descriptions in the operating instructions.
Error Cause Elimination
Droplets on the
inner wall of the
pipette tip. Uneven wetting of the plastic
wall.  Attach a new pipette tip.
Pipette is dripping,
pipetted volume is
incorrect. • Tip loose.  Press the tip on firmly.
• Incorrect pipette tip.  Use an original Eppendorf
tip.
Pipette is leaking because:
• The piston is
contaminated.
 Clean the piston and
lubricate slightly.
• The piston is damaged.  Replace the piston and
piston sealing and
lubricate slightly.
• The seal is damaged.  Replace the seal.