Eppendorf SOP - Manual (Page 6)

6
Test and pipette conditions
Eppendorf SOP
English (EN)
1 Test and pipette conditions
For the calibration or adjustment of pipettes, the scales and measuring station
should fulfill the following requirements:
1.1 Balances
1.1.1 Balance type
Use semi-microbalances and microbalances to calibrate pipettes.
Some companies offer balances that are specially designed to meet the
requirements of pipette calibration, e.g. Sartorius and Mettler.
1.1.2 Accuracy
When selecting the balances, ensure that they are suitable for the accuracy of
the pipette. This helps to ensure that deviations within a measuring series are
recorded exactly for the assessment of the systematic and random measurement
deviations in accordance with EN ISO 8655.
For a pipette volume of less than or equal to 10 μl, balances with a 6-digit
display must be used. With larger volumes, balances with a 5-digit display are
sufficient.
1.1.3 Minimum requirements for balances
* For practical reasons, the nominal volume can be used for the selection of the
balances.
These test and pipetting conditions are valid for all Eppendorf pipettes
and dispensers.
Follow EN ISO 8655 "Volume-measuring equipment with pistons".
Selected volume* of the pipette to be
tested Display resolution (mg)
1 μL to 10 μL 0.001
>10 μL to 100 μL 0.01
>100 μL to 1 000 μL 0.1
>1 mL to 10 mL 0.1
>10 mL to 200 mL 1