Eppendorf SOP - Manual (Page 17)

17
Adjustment
Eppendorf SOP
English (EN)
7 Adjustment
7.1 General information
Prior to delivery, all pipettes are adjusted with distilled or de-ionized, degassed
water under the conditions described in Chapters 1 to 3 and according to ISO
3696 (see Calibration on p. 8).
If you have doubts regarding the accuracy of the volume entered, please check
the following points first:
• Is there a leak in the device (see Leakage check on p. 15)?
Exception: Biomaster
• Does the temperature of the pipetted liquid correspond to:
– the temperature of the device?
– the ambient air temperature?
• Is the set volume correct?
• Has the liquid density and air pressure been taken into consideration?
• Does the density of the pipetted liquid deviate from the double-distilled,
degassed water?
• Was the work completed correctly, as described in the "Calibration" chapter
(see p. 8) and "Evaluation" chapter (see p. 11)?
• Were original Eppendorf pipette tips used?
Volume errors can also occur when pipetting liquids with high vapor pressure,
where the density or viscosity deviates considerably from the values of water.
Adjustments should only be carried out on the device after these conditions have
been taken into consideration.
7.2 Adjusting adjustable-volume pipettes
The adjustment of the following adjustable-volume pipettes is a zero point offset:
• Research
• Research plus
• Reference
• Biomaster
• Reference2
Observe the operating instructions for your pipette. There you will find
detailed descriptions on adjustment.