Eppendorf SOP - Manual (Page 18)

18
Adjustment
Eppendorf SOP
English (EN)
7.2.1 Procedure
Requirements
Device, original Eppendorf tip, test liquid and ambient air must have the same
temperature (15 – 30°C) at a constant temperature of ±0.5°C during the test
(according to EN ISO 8655, Part 6).
1. Set the pipette to the smallest volume to be tested.
2. Use a matching original Eppendorf tip for single-channel pipettes. For
Multi-channel pipettes, use any channel.
3. Pipette the set volume 10 times.
4. Conduct a weighing after every volume dispensing.
5. Calculate the mean value after 10 measurements (see Evaluation on p. 11).
The calculated mean value of these weighings (observe conversion factor Z =
mg to μL) yields the actual volume.
6. For adjusting, introduce the suitable tool into the opening intended for this
purpose, or place it on the designated location, and adjust the actual volume
(see the following figures or operating manual).
7. Check the set volume by measuring again. If the target volume does not
match the result of the measurement, repeat steps 2 to 6.
8. After the adjustment, check the measured values for accuracy at 50% of the
nominal volume and check the nominal volume for accuracy.
If your Research, Research plus or Reference has an adjustment, remove
it before carrying out the adjustment.
After adjusting, lock the adjustment opening with a new adjustment
seal.
Adjustment seals can be reordered (see operating manual).