Eppendorf SOP - Manual (Page 7)

7
Test and pipette conditions
Eppendorf SOP
English (EN)
1.2 Measuring station
1.2.1 Evaporation
Take evaporation protection into account while carrying out the measurement.
With volumes < 50 μl in particular, errors caused by the evaporation of the test
liquid must be taken into consideration.
This can be ensured by using a liquid trap or other equipment to prevent
evaporation.
1.2.2 Measuring station
For electronic measurement data processing, we recommend calibration
software and accessories for your balance (PICASO, order no. 3113 004.001).
(see also EN ISO 8655, Part 6)
1.2.3 Test room
The tests should be carried out in a draft-free room under constant climatic
conditions.
The test room should have a constant temperature between 15 °C and 30 °C and
a constant relative humidity above 50 %.
1.2.4 Temperature differences
Before the test, the device to be tested and test liquid must have stood in the test
room for a sufficient amount of time, at least 2 hours, in order to reach
equilibrium with the storage conditions.
Direct sunlight and other influences which could affect the temperature should
be avoided at all costs.
1.2.5 Test liquid
Distilled or deionized water of "Quality 3 in accordance with ISO 3696",
degassed or at equilibrium with air. The water must be at room temperature.
1.2.6 Operating Manual
Observe the operating instructions for your pipette.