Ocean Optics OOIBase32 - Manual (Page 97)

Appendix A
Experiment Tutorials
Overview
The following sections contain information on conducting sample experiments using the USB2000
Spectrometer and OOIBase32.
For information on experiments with Ocean Optics spectrometers other than the USB2000, consult the
operating instructions for your particular spectrometer model.
Preparing for Experiments
► Procedure
Follow the steps below to configure the USB2000 and OOIBase32 for experiments:
1. Verify that you have correctly installed the USB2000, installed OOIBase32, and configured the
light source and other sampling optics.
2. Open the OOIBase32 application, select Spectrometer | Configure from the menu bar, and
double-check that A/D Interface settings are correct.
3. Check your spectrometer setup configurations in OOIBase32:
Locate the Wavelength Calibration Data sheet that came with the USB2000. Select Spectrometer
| Configure from the menu and choose the Wavelength Calibration page. Ensure the First
Coefficient, Second Coefficient, Third Coefficient and Intercept correspond to those of the
system. If not, enter the values as listed on the Wavelength Calibration Data sheet.
4. Adjust the acquisition parameters using the Acquisition Parameters dialog bar or select
Spectrum | Configure Data Acquisition from the menu.
If you followed the previous steps and started OOIBase32, the spectrometer is already acquiring data.
Even with no light in the spectrometer, OOIBase32 should display a dynamic trace in the bottom of the
graph window. If you allow light into the spectrometer, the graph trace should rise with increasing light
intensity. This indicates that you correctly installed the software and hardware.
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