Ocean Optics OOIBase32 - Manual (Page 65)

7: Spectrometer Menu Functions
► Procedure
Perform the steps below to monitor a reference using the Integrated Intensity option:
Hardware Configuration:
1. Attach a fiber from the light source to the sample.
2. Attach a fiber from the sample to the spectrometer.
Software Configuration:
1. Open OOIBase32 and select Spectrometer | Configure.
2. Select the Reference Monitoring tab.
3. Locate the channel (in the Spectrometer Channel field) that you will use for your experiment.
4. Select the Reference Monitoring tab and select a Reference Channel.
5. Select Integrated Intensity under Reference Type.
6. Choose the Start and End for the Reference Bandwidth.
The software will use the wavelength area between these two points as the monitoring region.
You must know if the region you choose as the reference area is in a non-absorbing region of the
sample (during absorbance measurements) or is in the 100% transmission or reflectivity region of
the sample (during transmission or reflection measurements).
7. Store a dark and a reference spectrum of your sample while in Scope mode.
Note
You must perform Step 7 after configuring reference monitoring.
8. Change to the appropriate spectral view mode in OOIBase32 (or stay in Scope mode).
OOIBase32 now will automatically correct for drift and reflect any correction in the spectral window.
Single Point
If you cannot perform the dual-beam type of reference monitoring, you can use the Single Point method.
However, it is only possible for the software to correct for drift using this option if the drift from the
detected light is uniform. Choose this option over the Integrated Intensity option only if you cannot afford
to use as your reference an area of the wavelength region and must monitor a single wavelength point.
Use the Single Point option if:
• You do not have two spectrometer channels identically configured
• The drift of the light source is uniform
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