Ocean Optics OOIBase32 - Manual (Page 101)

A: Experiment Tutorials
Transmission Experiments
Transmission is the percentage of energy passing through a sample relative to the amount that passes
through the reference. Transmission mode can also display the portion of light reflected from a sample,
since transmission and reflection measurements use the same mathematical calculations. We express
transmission as a percentage (%T
λ ) relative to a standard substance (such as air). OOIBase32 calculates
%T λ (or %R λ ) with the following equation.
S λ - D λ
%T λ =
R λ - D λ
x 100%
Where:
S
λ = Sample intensity at wavelength
λ
D
λ = Dark intensity at wavelength
λ
R
λ = Reference intensity at wavelength
λ
Typical transmission setup: The light source (far right) sends light via the input leg of a transmission
probe into a container (bottom center). The light interacts with the sample. The output leg of the
transmission probe carries the information to the spectrometer (top center), which transmits the
information to the PC (far left).
Common transmission applications include measuring light through solutions, optical filters, optical
coatings, and other optical elements (such as lenses and fibers).
► Procedure
Perform the following steps to take a transmission measurement using OOIBase32:
1. Place OOIBase32 in scope mode by clicking the Scope Mode icon on the toolbar or by selecting
Spectrum | Scope Mode from the menu bar.
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