Ocean Optics OOIBase32 - Manual (Page 84)

8: Spectrum Menu Functions
Store Reference Dark
This option stores a reference dark spectrum for all enabled spectrometer channels in the active spectral
window while in time normalized intensity mode. Take the reference dark spectrum with the light path
blocked and with the integration time set to the value used when acquiring the reference spectra.
You must store a reference dark spectrum before OOIBase32 can calculate absorbance, transmission, and
relative irradiance spectra in the Time Normalized Intensity mode.
Select Spectrum | Time Normalized Intensity | Store Reference Dark.
Store Sample Dark
This option stores a sample dark spectrum for all enabled spectrometer channels in the active spectral
window while in time normalized intensity mode. Take the sample dark spectrum with the light path
blocked and with the integration time set to the value used when acquiring the sample spectra.
You must store a sample dark spectrum before OOIBase32 can calculate absorbance, transmission, and
relative irradiance spectra in the Time Normalized Intensity mode.
Select Spectrum | Time Normalized Intensity | Store Sample Dark.
Store Reference (Ctrl+R)
This option stores a reference spectrum for all enabled spectrometer channels in a spectral window while
in time normalized intensity mode. Take this spectrum with the light source on and a blank in the
sampling region.
You must store a reference spectrum before OOIBase32 can calculate absorbance, transmission, and
reflection spectra in the Time Normalized Intensity mode.
Select Spectrum | Time Normalized Intensity | Store Reference.
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