Ocean Optics OOIBase32 - Manual (Page 106)

A: Experiment Tutorials
► Procedure
Perform the following steps to take a relative irradiance measurement using OOIBase32:
1. Place OOIBase32 in scope mode by clicking the Scope Mode icon on the toolbar, or by selecting
Spectrum | Scope Mode from the menu bar.
2. Ensure that the entire signal is on scale. The intensity of the reference signal should peak at about
3500 counts.
Note
You must use a light source that is a blackbody of known color temperature.
3. In the Reference Color Temperature dialog box, enter the color temperature of the light source
(in Kelvin) and click the OK button.
4. Take a reference spectrum using a light source with a black body of a known color temperature,
such as the LS-1.
Click the Store Reference spectrum icon on the toolbar or select Spectrum | Store Reference
from the menu bar to store the reference. This command merely stores a reference spectrum in
memory. You must select File | Save | Reference from the menu bar to permanently save the
spectrum to disk.
5. Block the light path to the spectrometer. Then, take a dark spectrum by clicking the Store Dark
Spectrum icon on the toolbar or by selecting Spectrum | Store Dark from the menu bar. This
command merely stores a dark spectrum in memory. You must select File | Save | Dark from the
menu to permanently save the spectrum to disk.
Note
If possible, do not turn off the light source when taking a dark spectrum. If you must turn
off your light source to store a dark spectrum, allow enough time for the lamp to warm up
again before continuing your experiment.
You must take a dark spectrum before measuring relative irradiance.
6. Position the fiber at the light source you wish to measure. Then, choose the Irradiance mode
icon on the toolbar or select Spectrum | Relative Irradiance Mode from the menu bar.
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