Eppendorf Galaxy CO2 Sensor - Manual (Page 83)

Equipment options
Galaxy ® 170 R/170 S CO
2
Incubators
English (EN)
83
12.6 O
2
control (1 - 95 %) (Galaxy R only)
This oxygen control option uses the controlled addition of oxygen to increase the level above ambient and
the controlled addition of nitrogen to reduce the oxygen level below ambient. If you have the 1 - 19 %
oxygen control option, (see
O
2
control (1 - 19 %) (Galaxy R only) on p. 72); or for the 0.1 - 19 % oxygen
control option, (see O
2
control (0.1 - 19 %) (Galaxy R only) on p. 80).
If your incubator is operating in the 3 - 95 % range of O
2
control, only the N
2
valve will operate for
setpoints below 19 %; both the N
2
and O
2
valves will operate for setpoints between 19 and 80 %; and for
setpoints above 80 %, only the O
2
valve will operate.
12.6.1 Setting up the N
2
tank
Prerequisites
Before you set up your oxygen control, ensure that you have the proper equipment for your nitrogen
supply:
• 2 cylinders of nitrogen, regulation size W
• 1 two-stage pressure regulator
• 1 Inline pressure regulator
• 6 mm PVC tubing
• tubing clips
Set up the nitrogen tanks as follows:
1. Inspect them to ensure there are no leaks or other damage.
2. Check that the two-stage pressure regulator valve and the inline pressure regulator valve are closed by
trying to turn their knobs in a counter-clockwise direction; the knobs should be at the stop point.
3. Place the inline pressure regulator close to the incubator.
WARNING! Risk of personal injury
High oxygen levels inside the chamber require additional safety precautions:
 Setting the oxygen to levels > 24 % can greatly increase the chance of fire and explosion.
Operators planning to set oxygen at 24 - 95 % should therefore take all appropriate
precautions to minimize the risk. Eppendorf does not recommend using external
(heat-generating) equipment inside the chamber when oxygen conditions will be > 24 %.
 Before opening the incubator door, make sure no flames (Bunsen burner, etc.) are in the
vicinity.
 Avoid the presence of other combustible gases (hydrogen, methane, etc.)
 Ensure that all solenoid valves and pressure regulators and gauges controlling the gas
supply are grease-free for use with oxygen.
If you plan to maintain O
2
levels of 80 - 95 %, (see Important notes for O
2
levels > 80 % on
p. 85).