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5 co2 sampling with analyzer, Sampling with analyzer, Routine checks of the o – Eppendorf Galaxy CO2 Sensor User Manual

Page 52: Control option (170 r only)

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Maintenance
Galaxy

®

170 R/170 S CO

2

Incubators

English (EN)

52

If required, you can take a sample of the gas inside the chamber using the CO

2

sample port, and check it

using a CO

2

gas analyzer (see

Accessories on p. 63)(see CO

2

Sampling with analyzer on p. 52).

8.1.5

CO

2

Sampling with analyzer

The CO

2

sample port is located on the left-hand side of the incubator, near the top (see Fig. 5-1 on p. 29).

If you conduct a sampling, please ensure the following:

• Turn off the CO

2

gas by re-programming the setpoint for CO

2

to 0.0 % to prevent CO

2

from being

injected into the chamber and giving a false reading.

• A flow rate ≤ 0.5 liters/minute is used to take a sample.
• The door is kept closed.
• Reset the CO

2

setpoint to the desired level after sampling.

8.1.6

Routine checks of the O

2

control option (170 R only)

Be sure to conduct the reference to atmospheric oxygen procedure on a monthly basis to ensure that
long-term drift in output from the sensor will be corrected, and to determine when the sensor requires
replacement.

Under normal humidity conditions (95 - 99 % RH), the sensor is unaffected. If for any reason (such as a
large spill inside the chamber or the incubator being switched off while fully humidified) liquid condenses
around the sensor, the result may be restriction of gas flow and a low sensor signal. Should such
condensation appear on the chamber walls, normal operation can be easily restored by removing the
humidity tray, drying the chamber completely, and then running the incubator at 37 °C for one hour. This
will dry out the sensor. After the hour has elapsed, the humidity tray can be reinstalled and the incubator
humidified again.

Change the hydrophobic filter

each time you clean the incubator, to avoid contamination or clogging over

time.

Displayed chamber CO

2

level will drop during sampling, but it will recover once the sampling

is complete. This is merely a sensor characteristic; the CO

2

level in the chamber is actually not

affected.
We recommend that you perform a CO

2

Auto-Zero prior to sampling. We also recommend that

you Auto-Zero the CO

2

system at least once every 28 days to ensure that CO

2

level is correct.

We recommend that you perform a CO

2

Auto-Zero prior to sampling. We also recommend that

you Auto-Zero the CO

2

system at least once every 28 days to ensure that CO

2

level is correct.