Eppendorf Galaxy CO2 Sensor - Manual (Page 30)

Operation
Galaxy
® 170 R/170 S CO
2
Incubators
English (EN)
30
7. Leave the incubator running for at least two hours (preferably overnight) to allow conditions to stabilize.
5.2 Using the humidity tray
If humidification is required:
1. Fill the humidity tray with 1.5 - 2.5 liters of warm (37.0 °C) distilled water.
2. For cell culture work, we recommend adding copper sulphate in the humidity tray. Tests have shown
that, in addition to inhibiting bacterial growth in the tray, this can reduce contamination on the chamber
walls. Add one small teaspoonful (~0.5 g) of copper sulphate to the water in the humidity tray.
3. For sensitive work, we do not recommend the use of any biocide in the humidity tray. To reduce the
possibility of contamination, every 10 to 14 days, empty the tray, clean it with a solution of 70 %
isopropyl alcohol and 30 % distilled water, and then refill it with 1.5 liters of warm distilled water.
5.3 Operation for optional features
For functionality on optional features (see Available options on p. 64).
• The incubator’s CO
2
valve is disabled until the incubator reaches the temperature setpoint.
After the temperature setpoint is reached, the CO
2
valve is activated, allowing the
incubator to reach the CO
2
setpoint.
• If power is interrupted to the incubator long enough for the temperature to drop below
setpoint, the CO
2
valve will be deactivated until temperature setpoint is again achieved.
(This serves to avoid spurious CO
2
readings while the incubator is below its temperature
setpoint)
NOTICE! Risk of material damage
 To avoid possible damage to the CO
2
sensor, never leave water in the humidity tray while
the incubator is switched off, or when a high temperature disinfection cycle is initiated
(optional feature).
 Allow a clearance of 50 mm (2 in) to allow access for oxygen sensor (if installed) removal.
• The humidity tray should be left in place at all times.
• Use distilled water only in the humidity tray. Use of any other types of water including
deionized water will cause corrosion inside the incubator.
The humidity level within the chamber is not adjustable. The internal chamber will reach ~95
% relative humidity at 37 °C using the 1.5-liter humidity tray.